RESOURCES

Equipment

Cryo-EM laboratory equipment

jeol_jem_1400

 

JEOL JEM-1400

Transmission electron microscope operating at accelerating voltage of 120 kV  equipped with LaB6 cathode and TVIPS TemCam-F416 camera

 

 

 

TEMCAM-F416

 TVIPS CMOS TemCam-F416

High sensitivity 4016×4016 pixel CMOS camera of 2d generation combined with   software for  automated image collection allows using the microscope for collection hundreds of high quality  images automatically.

 

ELMO Glow discharge system

263-231-elmosmallThis instrument allows treatment of carbon grids with plasma. This cleans grid’s surface and renders it hydrophilic enhancing absorption of proteins on the surface of carbon.

 

Cryo transfer holder Gatan-626 Cryo-transfer-626

This sample holder is cooled to liquid nitrogen temperature. It allows transferring electron microscopy grids inside of the electron microscope and to take images of protein samples vitrified in thin layer of amorphous ice at cryogenic temperature (cryo-EM).

 Automated cryo-plunger 

plunger-dec-2014

We have a home-made cryo-pluner with temperature controlled ethane and sample compartments  as well with humidity control.

High-resolution single particle EM

FEI_TitanKrios

We have access to NeCEN for acquisition of high-resolution data on Titan Krios microscopes equipped with direct electron detector.

computer cluster

The high-resolution single particle datasets are processed on HYDRA – a high-performance computer cluster of ULB processing of high-resolution datasets is

Biochemistry lab

The laboratory of Structural Biology Research Centre of VIB/VUB  is equipped with the state of the art instruments for  protein purification,  biochemical and biophysical protein characterizatino:

  • Äkta systems
  • Ultracentrifuge
  • UV-Vis Spectrometers
  • Fluorimeters
  • CD spectrometer
  • ITC, etc.

SBRC also have in house X-ray generator, SAXS and NMR spectrometers for structural analysis of proteins.

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