Cryo-EM laboratory equipment
Transmission electron microscope operating at accelerating voltage of 120 kV equipped with LaB6 cathode and TVIPS TemCam-F416 camera
TVIPS CMOS TemCam-F416
High sensitivity 4016×4016 pixel CMOS camera of 2d generation combined with software for automated image collection allows using the microscope for collection hundreds of high quality images automatically.
ELMO Glow discharge system
This sample holder is cooled to liquid nitrogen temperature. It allows transferring electron microscopy grids inside of the electron microscope and to take images of protein samples vitrified in thin layer of amorphous ice at cryogenic temperature (cryo-EM).
We have a home-made cryo-pluner with temperature controlled ethane and sample compartments as well with humidity control.
High-resolution single particle EM
We have access to NeCEN for acquisition of high-resolution data on Titan Krios microscopes equipped with direct electron detector.
The high-resolution single particle datasets are processed on HYDRA – a high-performance computer cluster of ULB processing of high-resolution datasets is
The laboratory of Structural Biology Research Centre of VIB/VUB is equipped with the state of the art instruments for protein purification, biochemical and biophysical protein characterizatino:
- Äkta systems
- UV-Vis Spectrometers
- CD spectrometer
- ITC, etc.
SBRC also have in house X-ray generator, SAXS and NMR spectrometers for structural analysis of proteins.