Access to High-resolution single particle EM facilities
Until cryo-ARM300 is operational at VUB we have access to NeCEN, eBIC and other multiuser cryo-EM facilities for acquisition of high-resolution data on Titan Krios microscopes equipped with direct electron detector.
Transmission electron microscope operating at accelerating voltage of 120 kV equipped with LaB6 cathode
TVIPS CMOS TemCam-F416
High sensitivity 4016×4016 pixel CMOS camera of 2d generation combined with software for automated image collection allows using the microscope for collection hundreds of high quality images automatically.
ELMO Glow discharge system
This sample holder is cooled to liquid nitrogen temperature. It allows transferring electron microscopy grids inside of the electron microscope and to take images of protein samples vitrified in thin layer of amorphous ice at cryogenic temperature (cryo-EM).
with temperature controlled ethane and sample and automated humidity control.
The high-resolution single particle datasets are processed on HYDRA – a high-performance computer cluster of VUB
Microfluidics chip fabrication
Programmable hot plate
Our department is equipped with the state of the art instruments for protein purification, biochemical and biophysical protein characterizatino:
- Äkta systems
- UV-Vis Spectrometers
- CD spectrometer
- ITC, etc.
SBRC also have in house X-ray generator, SAXS and NMR spectrometers for structural analysis of proteins.